You're looking for a JSON to VCF (Variant Call Format) converter and an informative paper on the topic. Here's some information:
# Load JSON data with open('input.json') as f: data = json.load(f) json to vcf converter
vcf_writer.close() Note that this example assumes a simple JSON structure with a list of variants, each containing chr , pos , ref , and alt fields. You're looking for a JSON to VCF (Variant